中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (1): 101-108.DOI: 10.3969/j.issn.10017216.2012.01.016

• 综述与专论 • 上一篇    下一篇

水稻拔节期叶片碳氮代谢基因型差异及与氮素利用效率的关系

程建峰1,* ,戴廷波2 ,蒋海燕1,潘晓云1,曹卫星2   

  1. 1江西农业大学  教育部作物生理生态与遗传育种重点实验室, 江西  南昌 330045； 2南京农业大学  农业部作物生长调控重点开放实验室，  江苏  南京  210095；
  • 收稿日期:2011-04-09 修回日期:2011-07-28 出版日期:2012-01-10 发布日期:2012-01-10
  • 通讯作者: 程建峰1,*
  • 基金资助:

    国家863计划资助项目(2003AA209030)； 国家自然科学基金资助项目(30030090)。

Characterization of Leaf Carbon and Nitrogen Assimilation in Different Rice Genotypes at Jointing Stage and Their Relationships with Nitrogen Utilization Efficiency

CHENG  Jianfeng 1, * ， DAI Tingbo 2 ， JIANG Haiyan 1， PAN Xiaoyun 1， CAO Weixing 2   

  1. 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China; 2 Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;
  • Received:2011-04-09 Revised:2011-07-28 Online:2012-01-10 Published:2012-01-10
  • Contact: CHENG Jianfeng1, *

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被引次数

25

摘要： 采用土培盆栽试验，以3个氮素利用效率(NUE)有显著差异的水稻基因型秀恢2号(低NUE)、R8312(中NUE)和五优244(高NUE)为材料，研究了拔节期叶片碳氮代谢的基因型差异及其与氮素高效利用的关系。结果表明，拔节期不同基因型水稻的叶片蔗糖和可溶性糖含量、淀粉/可溶性糖含量比和淀粉/蔗糖含量比存在极显著差异，前两者为五优244 > R8312 >秀恢2号，后两者则相反；而淀粉和蔗糖/可溶性糖含量比各基因型差异不显著。不同基因型水稻的叶片全氮含量(TNC)、蛋白氮含量(PNC)、 PNC/TNC、Rubisco含量(RC)/PNC和RC/TNC存在极显著或显著差异，前两者为五优244 < R8312 <秀恢2号， 后三者则相反；RC无基因型差异。五优244叶片的叶绿素a含量、类胡萝卜素含量、PSⅡ潜在活性、羧化效率、碳酸酐酶、酸性转化酶和蔗糖磷酸合成酶活性极显著或显著高于其他基因型；其叶片的硝酸还原酶、谷氨酰胺合成酶、谷氨酸合成酶和谷氨酸脱氢酶活性也极显著高于其他基因型。统计分析表明，高NUE水稻叶片具有较高的可溶性糖和蔗糖含量及较大的RC/TNC和RC/PNC，其生理基础是其叶片的高谷氨酰胺合成酶活性和PSⅡ潜在活性。

关键词: 水稻, 碳代谢, 氮代谢, 氮素利用效率, 拔节期, 基因型

Abstract: The leaf carbon and nitrogen metabolism characteristics and their relationships with  nitrogen utilization efficiency(NUE) at  jointing stage were investigated with Xiuhui 2 (low NUE), R8312 (medium NUE) and Wuyou 244 (high NUE) as materials  under the lower nitrogen level (58 mg/kg). The results showed that the contents of sucrose and soluble sugar  in the leaves of different rice genotypes    were Wuyou 244 > R8312 > Xiuhui 2, the ratio  of starch content to soluble sugar content and  the ratio of starch  content to sucrose  content  were Xiuhui 2>R8312>Wuyou 244, significantily differed among genotypes. The  starch content and the  ratio of sucrose content to soluble sugar content were not genotypically specific. The  total nitrogen content (TNC), protein nitrogen content (PNC), PNC/TNC, RC(Rubisco content)/PNC  and RC/TNC in the leaves of different rice genotypes exhibited significant differences,  showing an increasing trend of Wuyou 244 < R8312 < Xiuhui 2  for the former two indices and a trend of Xiuhui 2>R8312>Wuyou 244 for the latter  three; RC was not genotypicallyspecific. The contents of chlorophyll and carotenoid, activity of PSⅡ, carboxylation efficiency, activities of carbonic anhydrase, acid invertase and sucrose phosphate synthase in the leaves of Wuyou 244 with high NUE were significantly higher than those of the other genotypes. The activities of nitrate reductase, glutamine synthetase and glutamate synthase and glutamate dehydrogenase in the leaves of Wuyou 244 with high NUE were also significantly higher than those of the other genotypes. Statistical analyses indicated that the leaves of rice genotype with high NUE had the distinguished characteristics of higher soluble sugar content, sucrose content, RC/TNC and RC/PNC, their physiological base were higher   glutamine synthetase activity and primary PSⅡ activity.

Key words: rice, carbon metabolism, nitrogen metabolism, nitrogen use efficiency(NUE), jointing stage, genotype

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